Abstract
Radioactive vitamin E (D-α-[5-methyl-3H]tocopherol) bound with the cytosol (100,000g supernatant), pellet (100,000g pellet), crude nuclear, and purified chromatin fractions from mouse neuroblastoma (NBP2) and rat glioma (C-6) cells in culture.
The level and type of vitamin E binding proteins in the cytosol depended upon the cell type.
When the cytosol proteins containing radioactive vitamin E were separated by gel filtration (Sepharose 4B gel), there were five protein peaks in neuroblastoma, three peaks in glioma, and one peak in mouse B-16 melanoma cells which contained bound radioactivity.
The level of binding in the neuroblastoma cells was higher than that in glioma cells or melanoma cells.
Vitamin E remained bound to the proteins from the cytosols of neuroblastoma and glioma even after denaturation and separation by electrophoresis.
This suggests that vitamin E is tightly bound with the cytosol proteins.
There was only one vitamin E binding protein in the pellet and nuclear fractions of NB, glioma, and melanoma cells. The significance of vitamin E binding proteins in the mechanism of the effect of vitamin E on mammalian cells in culture is unknown.
See also:
- Solution of retinoids in vitamin E in the Di Bella Method biological multitherapy;
- All-Trans-Retinoic Acid (ATRA - analogues and/or derivatives);
- Neuroblastoma: Complete objective response to biological treatment;
- Complete objective response to biological therapy of plurifocal breast carcinoma.