Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas

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Published on Wednesday, 09 October 2019

Abstract

A malignant Nb rat lymphoma which in vivo is stimulated by estrogens has been established in suspension culture.

The cultured cells grew readily in Fischer's medium supplemented with fetal calf serum (10%) and 2-mercaptoethanol (10(-4) M).

If horse serum was substituted for fetal calf serum, population growth ceased; i.e., cultures became "stationary."

Such stationary cultures could be induced to resume active growth by the addition of a pituitary hormone, prolactin (ovine, rat); concentrations as low as 10 pg/ml had a detectable effect.

In contrast, other pituitary hormones or estrogens had little or no effect. The evidence in this and an accompanying paper suggests that prolactin (or related substances) has a role in the growth of some cancers of lymphoid origin in rats.

 

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- Publication: The Entrapment of Somatostatin in a Lipid Formulation: Retarded Release and Free Radical Reactivity (from Di Bella's Foundation);

- Cyclophosphamide plus Somatostatin, Bromocriptin, Retinoids, Melatonin and ACTH in the Treatment of Low-grade Non-Hodgkin’s Lymphomas at Advanced Stage: Results of a Phase II Trial;

- Relapse of High-Grade Non-Hodgkin’s Lymphoma After Autologous Stem Cell Transplantation: A Case Successfully Treated With Cyclophosphamide Plus Somatostatin, Bromocriptine, Melatonin, Retinoids, and ACTH;

- Low-grade Non-Hodgkin Lymphoma at Advanced Stage: A Case Successfully Treated With Cyclophosphamide Plus Somatostatin, Bromocriptine, Retinoids, and Melatonin;

- The Di Bella Method (DBM) improved survival, objective response and performance status in a retrospective observational clinical study on 55 cases of Lymphomas;

- Large B-cells Non-Hodgkin's Lymphoma, Stage IV-AE: a Case Report;

- Non-Hodgkin's Lymphoma, Stage III-B-E: a Case Report;

- The Synergism of Somatostatin, Melatonin, Vitamins Prolactin and Estrogen Inhibitors Increased Survival, Objective Response and Performance Status In 297 Cases of Breast Cancer;

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- Pancreatic Adenocarcinoma: clinical records on 17 patients treated with Di Bella's Method;

- The Di Bella Method Increases by the 30% the survival rate for Pancreas tumors and for this reason should be proposed as first line therapy for this type of cancer.