1,25-Dihydroxyvitamin D3 receptor in a cultured human breast cancer cell line (MCF 7 cells)

Summary

MCF 7, a cultured human breast cancer cell line, is known to resorb bone independently of osteoclast action.

In this report MCF 7 cells are shown to possess a receptor for 1,25-dihydroxyvitamin D3 with a low capacity (8,000 sites/cell) and high affinity (Kd 1.1*10−11M), and which sediments at 3.7S in sucrose density gradients.

These characteristics and the relative affinity of other vitamin D and non-vitamin D steroids are very similar to those found for the well characterised chicken intestinal 1,25-(OH)2D receptor.

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See also Vitamin D and cancer.